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  5. Ne Does Not Provide Sufficient Information on Allelic Variation: Suggestions to Fill the Gap.

Ne Does Not Provide Sufficient Information on Allelic Variation: Suggestions to Fill the Gap.

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Resource type
Journal article
Creator (person)
Hoban, Sean M.
ORCIDORCID logo
Forester, Brenna R.
Gargiulo, Roberta
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Koontz, Austin C.
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Mastretta‐Yanes, Alicia
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Mergeay, Joachim
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Paz‐Vinas, Ivan
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Laikre, Linda
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Date published
March 20, 2026
Abstract
Conservation success depends on translating theory into practical guidance and tools that are relevant and useful for non‐scientists. While the complexity of population genetics has challenged the usage of straightforward metrics for conservation, several practical guidelines have been advanced, such as those regarding effective population size ( ). Allendorf et al. highlight limitations of as a metric for practical use. Specifically, they demonstrate that while is sufficient for predicting heterozygosity, it is not predictive of the number of alleles, another key variable in conservation genetics. This has important implications for ‐based metrics, such as the 500 indicator recently adopted in the Convention on Biological Diversity's Kunming–Montreal Global Biodiversity Framework. As developers and advocates of the 500 indicator, we agree with this assessment, and acknowledge that does not comprehensively predict changes in allelic variation. In this article we briefly summarize several major points in Allendorf et al. and provide practical suggestions to better account for allelic variation during indicator assessments. These suggestions include reporting major declines in as part of genetic assessments, clearly articulating the intention and caveats of the 500 indicator, integrating simulations into genetic assessments, and assessing the number of genetically distinct populations. We conclude that the 500 indicator remains a valuable metric uniquely capable of capturing critical aspects of a species' genetic status while remaining accessible and interpretable to policymakers and other non‐geneticists. By acknowledging the limitations of focusing solely on and providing options for more thorough and nuanced understandings of genetic diversity, we hope to guide future usage of the 500 indicator and help bridge the gap between conservation genetics theory and practice.
Creator (organisation)
Coalition for Conservation Genetics
Project(s)
Priority 1: Ecosystem Stewardship
Funder
Funder nameAwards
European Cooperation in Science and Technology, Belgium
Walder Foundation, United States
Biota award
Vetenskapsrådet, Sweden
2019-05503 - 2025-04811
Svenska Forskningsrådet Formas, Sweden
2020-01290
Havs- och Vattenmyndigheten, Sweden
Dnr 2025-002371
Journal title
Evolutionary Applications
Volume
19
Issue
3
Article number
e70219
Publisher
John Wiley & Sons Ltd.
Place of publication
UK
ISSN
1752-4571
eISSN
1752-4571
Date accepted
February 25, 2026
Official URL
https://doi.org/10.1111/eva.70219
Rights statement
In Copyright
Licence
https://creativecommons.org/licenses/by/4.0/
DOI
10.1111/eva.70219
Keywords
Effective population size
Alleles
Ne 500 indicator
Allelic variation
Population size
Conservation genetics
Population genetics
Additional information
iF = 3.2 (2024)
Managed by the British Library and supported by the AHRC

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