A DNA barcoding framework for taxonomic verification in the Darwin Tree of Life Project.
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Resource type
Journal article
Creator (person)
Twyford, Alex D.
Beasley, Jordan
Barnes, Ian
Allen, Heather
Azzopardi, Freja
Bell, David
Blaxter, Mark L.
Broad, Gavin
Campos-Dominguez, Lucia
Choonea, Darren
Crowley, Liam
Cuber, Piotr
Cunliffe, Michael
Dombrowski, Alexandra
Douglas, Brian
Forrest, Laura L.
Gaya, Ester
Greeves, Clementine
Griffin, Claire
Harley, Joanna
Hart, Michelle L.
Holland, Peter W.H.
Hollingsworth, Peter M.
Januszczak, Inez
Jones, Amanda
Kersey, Paul
Kilias, Estelle
Lawniczak, Mara K.N.
Lewis, Owen T.
Mian, Sahr
Minotto, Alice
Misra, Raju
Mulhair, Peter O.
Pereira da Conceicoa, Lyndall
Price, Ben W.
Salatino, Silvia
Shaw, Felix
Sivell, Olga
Sivess, Laura
Uhl, Rebekka
Woof, Kieran
Date published
June 24, 2024
Abstract
Biodiversity genomics research requires reliable organismal identification, which can be difficult based on morphology alone. DNA-based identification using DNA barcoding can provide confirmation of species identity and resolve taxonomic issues but is rarely used in studies generating reference genomes. Here, we describe the development and implementation of DNA barcoding for the Darwin Tree of Life Project (DToL), which aims to sequence and assemble high quality reference genomes for all eukaryotic species in Britain and Ireland. We present a standardised framework for DNA barcode sequencing and data interpretation that is then adapted for diverse organismal groups. DNA barcoding data from over 12,000 DToL specimens has identified up to 20% of samples requiring additional verification, with 2% of seed plants and 3.5% of animal specimens subsequently having their names changed. We also make recommendations for future developments using new sequencing approaches and streamlined bioinformatic approaches.
Creator (organisation)
Darwin Tree of Life Consortium
Project(s)
Priority 2: Trait Diversity and Function
Priority 1: Ecosystem Stewardship
Funder
| Funder name | Awards |
Wellcome Trust, United Kingdom | 218328 |
Journal title
Wellcome Open Research
Volume
9
Article number
339
Publisher
Wellcome Trust
Place of publication
London, UK
eISSN
2398-502X
Date accepted
September 20, 2024
Official URL
Rights statement
In Copyright
Additional information
IF = 1.777 (2023-2024)