Paternal low protein diet perturbs inter-generational metabolic homeostasis in a tissue-specific manner in mice.
Name
s42003-022-03914-8.pdf
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3.91 MB
Format
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Resource type
Journal article
Date published
September 8, 2022
Abstract
The underlying mechanisms driving paternally-programmed metabolic disease in offspring remain poorly defined. We fed male C57BL/6 mice either a control normal protein diet (NPD; 18% protein) or an isocaloric low protein diet (LPD; 9% protein) for a minimum of 8 weeks. Using artificial insemination, in combination with vasectomised male mating, we generated offspring using either NPD or LPD sperm but in the presence of NPD or LPD seminal plasma. Offspring from either LPD sperm or seminal fluid display elevated body weight and tissue dyslipidaemia from just 3 weeks of age. These changes become more pronounced in adulthood, occurring in conjunction with altered hepatic metabolic and inflammatory pathway gene expression. Second generation offspring also display differential tissue lipid abundance, with profiles similar to those of first generation adults. These findings demonstrate that offspring metabolic homeostasis is perturbed in response to a suboptimal paternal diet with the effects still evident within a second generation.
Project(s)
Priority 2: Trait Diversity and Function
Funder
| Funder name | Awards |
Aston Research Centre for Healthy Ageing | Fellowship |
Biotechnology and Biological Sciences Research Council, United Kingdom | BB/R003556/1 - BB/M027252/1 |
Journal title
Communications Biology
Volume
5
Article number
929
Publisher
Springer Science and Business Media LLC
Place of publication
Berlin/Heidelberg, Germany
eISSN
2399-3642
Date accepted
August 30, 2022
Official URL
Rights statement
In Copyright
Additional information
IF = 5.489 (2021-2022)