Targeted sequencing supports morphology and embryo features in resolving the classification of Cyperaceae tribe Fuireneae s.l.

Starr, Julian R.; Jiménez‐Mejías, Pedro; Zuntini, Alexandre R.; Léveillé‐Bourret, Étienne; Semmouri, Ilias; Muasya, Muthama; Baker, William J.; Brewer, Grace E.; Epitawalage, Niroshini; Fairlie, Isabel; Forest, Félix; Sabino Kikuchi, Izai A.B.; Pokorny, Lisa; Larridon, Isabel

Targeted sequencing supports morphology and embryo features in resolving the classification of Cyperaceae tribe Fuireneae s.l.

Resource type

Journal article

Creator (person)

Starr, Julian R.

Jiménez‐Mejías, Pedro

ORCID

Zuntini, Alexandre R.

Léveillé‐Bourret, Étienne

ORCID

Semmouri, Ilias

Muasya, Muthama

Baker, William J.

Brewer, Grace E.

Epitawalage, Niroshini

Fairlie, Isabel

Forest, Félix

Sabino Kikuchi, Izai A.B.

Pokorny, Lisa

Larridon, Isabel

ORCID

Date published

January 14, 2021

Abstract

Molecular phylogenetic studies based on Sanger sequences have shown that Cyperaceae tribe Fuireneae s.l. is paraphyletic. However, taxonomic sampling in these studies has been poor, topologies have been inconsistent, and support for the backbone of trees has been weak. Moreover, uncertainty still surrounds the morphological limits of Schoenoplectiella, a genus of mainly small, amphicarpic annuals that was recently segregated from Schoenoplectus. Consequently, despite ample evidence from molecular analyses that Fuireneae s.l. might consist of two to four tribal lineages, no taxonomic changes have yet been made. Here, we use the Angiosperms353 enrichment panel for targeted sequencing in order to: (1) clarify the relationships of Fuireneae s.l. with the related tribes Abildgaardieae, Eleocharideae and Cypereae; (2) define the limits of Fuireneae s.s., and (3) test the monophyly of Fuireneae s.l. genera with emphasis on Schoenoplectus and Schoenoplectiella. Using more than a third of Fuireneae s.l. diversity, our phylogenomic analyses strongly support six genera and four major Fuireneae s.l. clades that we recognise as tribes: Bolboschoeneae stat.nov., Fuireneae s.s., Schoenoplecteae, and Pseudoschoeneae tr.nov. These results are consistent with morphological, micromorphological (nutlet epidermal cell shape), and embryo differences detected for each tribe. At the generic level, most sub‐Saharan African perennials currently treated in Schoenoplectus are transferred to Schoenoplectiella. Our targeted sequencing results show that these species are nested in Schoenoplectiella, and their treatment here is consistent with micromorphological and embryo characters shared by all Schoenoplectiella species. Keys to recognised tribes and genera are provided.