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  5. Putting small and big pieces together: a genome assembly approach reveals the largest Lamiid plastome in a woody vine.

Putting small and big pieces together: a genome assembly approach reveals the largest Lamiid plastome in a woody vine.

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Resource type
Journal article
Creator (person)
Fonseca, Luiz Henrique M.
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Nazareno, Alison G.
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Thode, Verônica A.
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Zuntini, Alexandre R.
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Lohmann, Lúcia G.
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Date published
April 7, 2022
Abstract
The plastid genome of flowering plants generally shows conserved structural organization, gene arrangement, and gene content. While structural reorganizations are uncommon, examples have been documented in the literature during the past years. Here we assembled the entire plastome of and compared its structure and gene content with nine other Lamiid plastomes. The plastome of is composed of 183,052 bp and follows the canonical quadripartite structure, synteny, and gene composition of other angiosperms. Exceptionally large inverted repeat (IR) regions are responsible for the uncommon length of the genome. At least four events of IR expansion were observed among the seven Bignoniaceae species compared, suggesting multiple expansions of the IRs over the SC regions in the family. A comparison with 6,231 other complete plastomes of flowering plants available on GenBank revealed that the plastome of is the longest Lamiid plastome described to date. The newly generated plastid genome was used as a source of selected genes. These genes were combined with orthologous regions sampled from other species of Bignoniaceae and all gene alignments concatenated to infer a phylogeny of the family. The tree recovered is consistent with known relationships within the Bignoniaceae.
Project(s)
Priority 4: Accelerated Taxonomy
Funder
Funder nameAwards
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brazil / Fundação de Amparo à Pesquisa do Estado de São Paulo, Brazil
2013/12633-8 - 2013/11706-1 - 2011/09160-5 - 2012/50260-6
Journal title
PeerJ
Volume
10
Article number
e13207
Publisher
PeerJ
Place of publication
Corte Madera, Calif., US
eISSN
2167-8359
Date accepted
March 10, 2022
Official URL
https://doi.org/10.7717/peerj.13207
Related URL
https://peerj.com/articles/13207
Rights statement
In Copyright
Licence
https://creativecommons.org/licenses/by/4.0/
DOI
10.7717/peerj.13207
Keywords
Inverted repeats (IRs)
Bignonia magnifica
Short- and long-reads sequencing
Plastome evolution
Additional information
IF = 2.984 (2021-2022)
Managed by the British Library and supported by the AHRC

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