Novel Insights into the Nature of Intraspecific Genome Size Diversity in Cannabis sativa L.
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plants-11-02736-v2.pdf
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1.84 MB
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Resource type
Journal article
Date published
October 16, 2022
Abstract
Cannabis sativa has been used for millennia in traditional medicine for ritual purposes and for the production of food and fibres, thus, providing important and versatile services to humans. The species, which currently has a worldwide distribution, strikes out for displaying a huge morphological and chemical diversity. Differences in Cannabis genome size have also been found, suggesting it could be a useful character to differentiate between accessions. We used flow cytometry to investigate the extent of genome size diversity across 483 individuals belonging to 84 accessions, with a wide range of wild/feral, landrace, and cultivated accessions. We also carried out sex determination using the MADC2 marker and investigated the potential of flow cytometry as a method for early sex determination. All individuals were diploid, with genome sizes ranging from 1.810 up to 2.152 pg/2C (1.189-fold variation), apart from a triploid, with 2.884 pg/2C. Our results suggest that the geographical expansion of Cannabis and its domestication had little impact on its overall genome size. We found significant differences between the genome size of male and female individuals. Unfortunately, differences were, however, too small to be discriminated using flow cytometry through the direct processing of combined male and female individuals.
Project(s)
WECANN
Funder
| Funder name | Awards |
Ministerio de Ciencia, Innovación y Universidades, Spain | CGL2017-84297-R - PRE2018-083226 |
Generalitat de Catalunya, Spain | Grant no. 2017SGR1116 |
Agencia Estatal de Investigación, Spain | Grant no. RYC-2014-16608 - Grant no. RYC-2017-2274 |
Cannaflos—Gesellschaft für medizinisches Cannabis mbH, Germany | |
Uriach Laboratories, Spain | |
Journal title
Plants
Volume
11
Issue
20
Article number
2736
Publisher
MDPI AG
Place of publication
Basel, Switzerland
eISSN
2223-7747
Date accepted
October 11, 2022
Official URL
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In Copyright
Additional information
IF = 3.899 (2021-2022)