RIP mutated ITS genes in populations of Ophiocordyceps sinensis and their implications for molecular systematics
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Resource type
Journal article
Creator (person)
Li, Yi
Jiang, Lan
Wang, Ke
Wu, Hai-Jun
Yang, Rui-Heng
Yan, Yu-Jing
Bushley, Kathryn E.
Hawksworth, David L.
Wu, Zujian
Yao, Yi-Jian
Date published
September 16, 2020
Abstract
Different hypotheses have been proposed to interpret the observed unusual ITS (internal transcribed spacer) sequences in Ophiocordyceps sinensis. The coexistence of diverged ITS paralogs in a single genome was previously shown by amplifying the ITS region from mono-ascospore isolates using specific primers designed for different ITS paralog groups. Among those paralogs, are AT-biased ITS sequences which were hypothesized to result from repeat-induced point mutation (RIP). This is a process that detects and mutates repetitive DNA and frequently leads to epigenetic silencing, and these mutations have been interpreted as pseudogenes. Here we investigate the occurrence and frequency of ITS pseudogenes in populations of O. sinensis using large-scale sampling, and discusses the implications of ITS pseudogenes for fungal phylogenetic and evolutionary studies. Our results demonstrate a wide distribution of ITS pseudogenes amongst different geographic populations, and indicate how ITS pseudogenes can contribute to the reconstruction of the evolutionary history of the species.
Funder
| Funder name | Awards |
National Natural Science Foundation of China | 30025002 - 31400018 |
Chinese Academy of Sciences | KSCX2-YW-G-074-04 - Scheme of Introduction of Overseas Outstanding Talents - KSCX2-YW-G-076 - KSCX2-SW-101C |
Ministry of Science and Technology of China | 2013BAD16B01 - 2012FY111600 - 2012CB126308 |
Journal title
IMA Fungus
Volume
11
Article number
18
Publisher
Springer Science and Business Media LLC
eISSN
2210-6359
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In Copyright