Potential chemopreventive, anticancer and anti-inflammatory properties of a refined artocarpin-rich wood extract of Artocarpus heterophyllus Lam.
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Resource type
Journal article
Date published
December 2021
Abstract
Colorectal cancer (CRC) represents the third leading cause of death among cancer patients below the age of 50, necessitating improved treatment and prevention initiatives. A crude methanol extract from the wood pulp of was found to be the most bioactive among multiple others, and an enriched extract containing 84% ( / ) artocarpin (determined by HPLC–MS–DAD) was prepared. The enriched extract irreversibly inhibited the activity of human cytochrome P450 CYP2C9, an enzyme previously shown to be overexpressed in CRC models. In vitro evaluations on heterologously expressed microsomes, revealed irreversible inhibitory kinetics with an IC value of 0.46 µg/mL. Time- and concentration-dependent cytotoxicity was observed on human cancerous HCT116 cells with an IC value of 4.23 mg/L in 72 h. We then employed the azoxymethane (AOM)/dextran sodium sulfate (DSS) colitis-induced model in C57BL/6 mice, which revealed that the enriched extract suppressed tumor multiplicity, reduced the protein expression of proliferating cell nuclear antigen, and attenuated the gene expression of proinflammatory cytokines ( and ) and protumorigenic markers ( , , , and . The extract significantly ( = 0.03) attenuated (threefold) the gene expression of murine , an enzyme homologous to the human CYP2C9 enzyme. These promising chemopreventive, cytotoxic, anticancer and anti-inflammatory responses, combined with an absence of toxicity, validate further evaluation of extract as a therapeutic agent.
Funder
| Funder name | Awards |
Graduate Studies and Research Grant | 00001 |
University of the West Indies Development Fund | 000122 |
HATCH | MAS00556 |
Caribbean Public Health Agency and National Cancer Institute | 62798 |
National Commission for Science and Technology, Jamaica | 00001 |
Journal title
Scientific Reports
Volume
11
Article number
6854
Publisher
Springer Science and Business Media LLC
Place of publication
Berlin/Heidelberg, Germany
eISSN
2045-2322
Date accepted
March 10, 2021
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