In vitro anti-HIV and cytotoxic effects of pure compounds isolated from Croton macrostachyus Hochst. Ex Delile.
Name
s12906-022-03638-6.pdf
Description
visibility:open
Size
1.74 MB
Format
Adobe PDF
Checksum (CRC64NVME)
Z55cZAXAHgc=
Resource type
Journal article
Date published
June 15, 2022
Abstract
is an important plant in traditional African medicine, widely utilized to treat a variety of diseases. In Kenya, HIV-infected patients use leaf and root decoctions of the plant as a cure for cough, back pain, bleeding, skin diseases, warts, pneumonia, and wounds. This study aimed to evaluate the anti-HIV activities and cytotoxic effects of extracts and chemical constituents isolated from In our previous study we demonstrated that the hexane, CH Cl , ethyl acetate and methanol soluble fractions of a 1:1 CH Cl /MeOH crude extracts of the leaves and stem bark of exhibited potent anti-HIV activities against HIV-1 with IC values ranging from 0.02–8.1 μg/mL and cytotoxicity effects against MT-4 cells ranging from IC = 0.58–174 μg/mL Hence, hexane soluble extract of 1:1 CH Cl /MeOH crude extract of the leaves of that was more potent against HIV-1 at IC = 0.02 μg/mL was subjected to column chromatography leading to the isolation of 2-methoxy benzyl benzoate (1), lupenone (2), lupeol acetate (3), betulin (4), lupeol (5), sitosterol (6) and stigmasterol (7). Lupenone (2), lupeol acetate (3) and betulin (4) exhibited anti-HIV-1 inhibition at IC = 4.7 nM, 4.3 and 4.5 μg/mL respectively. The results obtained from this study support the potential of as a source of anti-HIV constituents.
Project(s)
Priority 5: Enhanced Partnerships
Priority 2: Trait Diversity and Function
Funder
| Funder name | Awards |
United States International University Africa | 10-2854 |
Princess Nourah Bint Abdulrahman University, Saudi Arabia | Researchers Supporting Project number (PNURSP2022R62) |
King Saud University, Saudi Arabia | Researchers Supporting Project number (RSP-2021/26) |
Journal title
BMC Complementary Medicine and Therapies
Volume
22
Article number
159
Publisher
Springer Science and Business Media LLC
Place of publication
Berlin/Heidelberg, Germany
eISSN
2662-7671
Date accepted
June 2, 2022
Official URL
Rights statement
In Copyright
Additional information
IF = 3.78 (2021-2022)