Characterization of Terpenoids from the Ambrosia Beetle Symbiont and Laurel Wilt Pathogen Harringtonia lauricola.
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Resource type
Journal article
Date published
December 7, 2023
Abstract
Little is known concerning terpenoids produced by members of the fungal order Ophiostomales, with the member Harringtonia lauricola having the unique lifestyle of being a beetle symbiont but potentially devastating tree pathogen. Nine known terpenoids, including six labdane diterpenoids (1–6) and three hopane triterpenes (7–9), were isolated from H. lauricola ethyl acetate (EtOAc) extracts for the first time. All compounds were tested for various in vitro bioactivities. Six compounds, 2, 4, 5, 6, 7, and 9, are described functionally. Compounds 2, 4, 5, and 9 expressed potent antiproliferative activity against the MCF-7, HepG2 and A549 cancer cell lines, with half-maximal inhibitory concentrations (IC50s) ~12.54–26.06 μM. Antimicrobial activity bioassays revealed that compounds 4, 5, and 9 exhibited substantial effects against Gram-negative bacteria (Escherichia coli and Ralstonia solanacearum) with minimum inhibitory concentration (MIC) values between 3.13 and 12.50 μg/mL. Little activity was seen towards Gram-positive bacteria for any of the compounds, whereas compounds 2, 4, 7, and 9 expressed antifungal activities (Fusarium oxysporum) with MIC values ranging from 6.25 to 25.00 μg/mL. Compounds 4, 5, and 9 also displayed free radical scavenging abilities towards 2,2-diphenyl-1-picrylhydrazyl (DPPH) and superoxide (O2−), with IC50 values of compounds 2, 4, and 6 ~3.45–14.04 μg/mL and 22.87–53.31 μg/mL towards DPPH and O2−, respectively. These data provide an insight into the biopharmaceutical potential of terpenoids from this group of fungal insect symbionts and plant pathogens.
Project(s)
Priority 2: Trait Diversity and Function
Funder
| Funder name | Awards |
National Natural Science Foundation of China, China | No. 32270029 - No. U1803232 - No. 31670026 |
National Key Research and Development Program of China, China | No. 2017YFE0122000 |
Social Service Team Support Program Project, China | 11899170165 |
Fujian Agriculture and Forestry University, China | Science and Technology Innovation Special Fund (no. KFB23084) |
Fujian Provincial Major Science and Technology Project, China | 2022NZ029017 |
Fujian Provincial Department of Science and Technology, China | Key Project (no. 2020N5005) |
Young and Middle-aged Teacher Education Research Project of Fujian Province | JAT210075 |
National Science Foundation, United States | IOS-2241029 |
U.S. Department of Agriculture, United States | NIFA award 2019-05150 |
Journal title
Journal of Fungi
Volume
9
Issue
12
Article number
1175
Publisher
MDPI AG
Place of publication
Basel, Switzerland
eISSN
2309-608X
Date accepted
December 5, 2023
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In Copyright
Additional information
IF = 5.724 (2022-2023)