Novel peptaibiotics identified from Trichoderma clade Viride.

This study focuses on the peptaibiome produced by different species of belonging to clade : SZMC 28387 (CBS 979.70), cf SZMC 28007 (TUCIM 4886/IQ 191), cf SZMC 28390 (TUCIM 416/TUB F-597), cf SZMC 28391 (TUCIM 423/DAOM 230018), SZMC 28748 (IMI 206040), SZMC 28747 (TUCIM 2730) and cf SZMC 28005 (TUCIM 4882/IQ 11). We were able to identify new compounds with similarity to already known groups of peptaibiotics, as well as completely newly discovered compounds using high-performance liquid chromatography (HPLC) -mass spectrometry (MS). From the 367 peptaibiotics identified, 216 are peptaibols and 111 are lipopeptaibols. Out of all peptaibols, 55 are previously known, while 161 are newly discovered. The new peptaibol subgroups Strigosellin A, B and Dorothopsin A, B are introduced. Furthermore, besides 38 previously known lipopeptaibols, 73 new lipopeptaibol sequences, named Lipostrigosellins and Lipohamatins are also reported. In addition, 41 peptaibol-like compounds with unusual C-terminus were also found. Out of the 7 strains examined, 5 produced both peptaibols and lipopeptaibols, while 2 only peptaibols. The well-known compound, Trikoningin KA V (TRK-V) also produced by SZMC 28387 (CBS 979.70), was studied for its folding dynamics using accelerated molecular dynamics simulations (aMD) for understanding the plausible three-dimensional structures adopted by these peptaibols of clade . We observed a propensity to form kinked, right-handed helical structures when simulated in an aqueous environment.

Project(s)

Priority 4: Accelerated Taxonomy

Funder

Funder name	Awards
Nemzeti Kutatási Fejlesztési és Innovációs Hivatal, Hungary	TKP2021-EGA-28 - EKÖP-24-4SZTE-629 - EKÖP-24-4-SZTE-605
Magyar Tudományos Akadémia, Hubgary	KGYNK 2024
Hungarian Research Network	HUN-REN 2001007