Stomatal responses to carbon dioxide and light require abscisic acid catabolism in Arabidopsis
Resource type
Journal article
Creator (person)
Movahedi, Mahsa
Zoulias, Nicholas
Casson, Stuart A.
Sun, Peng
Liang, Yun-Kuan
Hetherington, Alistair M.
Gray, Julie E.
Chater, Caspar C. C.
Date published
February 12, 2021
Abstract
In plants, stomata control water loss and CO2 uptake. The aperture and density of stomatal pores, and hence the exchange of gases between the plant and the atmosphere, are controlled by internal factors such as the plant hormone abscisic acid (ABA) and external signals including light and CO2. In this study, we examine the importance of ABA catabolism in the stomatal responses to CO2 and light. By using the ABA 8′-hydroxylase-deficient Arabidopsis thaliana double mutant cyp707a1 cyp707a3, which is unable to break down and instead accumulates high levels of ABA, we reveal the importance of the control of ABA concentration in mediating stomatal responses to CO2 and light. Intriguingly, our experiments suggest that endogenously produced ABA is unable to close stomata in the absence of CO2. Furthermore, we show that when plants are grown in short day conditions ABA breakdown is required for the modulation of both elevated [CO2]-induced stomatal closure and elevated [CO2]-induced reductions in leaf stomatal density. ABA catabolism is also required for the stomatal density response to light intensity, and for the full range of light-induced stomatal opening, suggesting that ABA catabolism is critical for the integration of stomatal responses to a range of environmental stimuli.
Funder
| Funder name | Awards |
Leverhulme Trust | |
European Commission | 700867 |
Biotechnology and Biological Sciences Research Council | |
Journal title
Interface Focus
Volume
11
Issue
2
Publisher
The Royal Society
eISSN
2042-8901
Official URL
Rights statement
In Copyright