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  5. Genome characterization of brugmansia latent virus, a novel tobamovirus.

Genome characterization of brugmansia latent virus, a novel tobamovirus.

Resource type
Journal article
Creator (person)
Scott-Brown, Alison S.
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D’Elia, Tom
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Devey, Dion S.
Funderburk, Joseph E.
Adkins, Scott
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Date published
July 22, 2020
Abstract
A novel tobamovirus, brugmansia latent virus (BrLV), was discovered during a study of brugmansia (Brugmansia spp.) in the living collections held at the Royal Botanic Gardens, Kew. Here, we report the complete genome sequence of BrLV, which is 6,397 nucleotides long and contains the four open reading frames (RNA-dependent RNA polymerase, methyltransferase/helicase, movement, and coat proteins) typical of tobamoviruses. The complete genome sequence of BrLV shares 69.7% nucleotide sequence identity with brugmansia mild mottle virus (BrMMV) and 66.7 to 68.7% identity with other tobamoviruses naturally infecting members of the Solanaceae plant family. Phylogenetic analysis of the complete genome nucleotide sequence and the deduced amino acid sequences of the four tobamovirus proteins place BrLV in a subcluster with BrMMV within the Solanaceae-infecting tobamovirus subgroup as a new species.
Funder
Funder nameAwards
Royal Botanic Gardens, Kew, United Kingdom
Pilot Study Award
Indian River State College, United States
The Banack Family Partnership Endowed Teaching Chair in Agriculture
Journal title
Archives of Virology
Volume
169
Publisher
Springer Science and Business Media LLC
Place of publication
Berlin/Heidelberg, Germany
ISSN
0304-8608
eISSN
1432-8798
Date accepted
May 30, 2020
Official URL
http://dx.doi.org/10.1007/s00705-020-04718-z
Rights statement
In Copyright
DOI
10.1007/s00705-020-04718-z
Keywords
Brugmansia
Genome sequence
Brugmansia latent virus (BrLV)
Tobamovirus
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