Phylogenetic trends in TZ staining analysis of six deep dormancy seeds.
Name
phylogenetic-trends-in-tz-staining-analysis-of-six-deep-dormancy-seeds.pdf
Description
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563.07 KB
Format
Adobe PDF
Checksum (CRC64NVME)
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Resource type
Journal article
Date published
April 15, 2024
Abstract
The assessment of seed quality and physiological potential is essential in seed production and crop breeding. In the process of rapid detection of seed viability using tetrazolium (TZ) staining, it is necessary to spend a lot of labour and material resources to explore the pretreatment and staining methods of hard and solid seeds with physical barriers. This study explores the TZ staining methods of six hard seeds ( , , , , and ) and summarizes the TZ staining conditions required for hard seeds by combining the difference in fat content between seeds and the kinship between species, thus providing a rapid viability test method for the protection of germplasm resources of endangered plants and the optimization of seed bank construction. The TZ staining of six species of hard seeds requires a staining temperature above 35 °C and a TZ solution concentration higher than 1%. Endospermic seeds require shorter staining times than exalbuminous seeds. The higher the fat content of the seeds, the lower the required incubation temperature and TZ concentration for staining, and the longer the staining time. And the closer the relationship between the two species, the more similar their staining conditions become. The TZ staining method of similar species can be predicted according to the genetic distance between the phylogenetic trees, and the viability of new species can be detected quickly.
Project(s)
Priority 2: Trait Diversity and Function
Funder
| Funder name | Awards |
Jiangsu Provincial Department of Education, China | ‘Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)’ - Jiangsu Postgraduate Training Innovation Project, KYCX23_1234’ |
Jiangsu Forestry Science and Technology Innovation and Promotion Project, China | Project ‘LYKJ-NanJing[2022]01’ - Project ‘LYKJ [2021] 03’ |
Journal title
Seed Science Research
Publisher
Cambridge University Press (CUP)
Place of publication
Cambridge, UK
ISSN
0960-2585
eISSN
1475-2735
Official URL
Rights statement
In Copyright
Additional information
IF = 1.585 (2023-2024)