Phylogenetic Relationships Within the Hyper-Diverse Genus Eugenia (Myrtaceae: Myrteae) Based on Target Enrichment Sequencing.
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fpls-12-759460.pdf
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Resource type
Journal article
Date published
February 4, 2022
Abstract
is one of the most taxonomically challenging lineages of flowering plants, in which morphological delimitation has changed over the last few years resulting from recent phylogenetic study based on molecular data. Efforts, until now, have been limited to Sanger sequencing of mostly plastid markers. These phylogenetic studies indicate 11 clades formalized as infrageneric groups. However, relationships among these clades are poorly supported at key nodes and inconsistent between studies, particularly along the backbone and within sect. encompasses ca. 700 species. To resolve and better understand systematic discordance, 54 taxa were subjected to phylogenomic Hyb-Seq using 353 low-copy nuclear genes. Twenty species trees based on coding and non-coding loci of nuclear and plastid datasets were recovered using coalescent and concatenated approaches. Concordant and conflicting topologies were assessed by comparing tree landscapes, topology tests, and gene and site concordance factors. The topologies are similar except between nuclear and plastid datasets. The coalescent trees better accommodate disparity in the intron dataset, which contains more parsimony informative sites, while concatenated trees recover more conservative topologies, as they have narrower distribution in the tree landscape. This suggests that highly supported phylogenetic relationships determined in previous studies do not necessarily indicate overwhelming concordant signal. Congruence must be interpreted carefully especially in concatenated datasets. Despite this, the congruence between the multi-species coalescent (MSC) approach and concatenated tree topologies found here is notable. Our analysis does not support subg. or sect. , as currently circumscribed, suggesting necessary taxonomic reassessment. Five clades are further discussed within sect. progress toward its division into workable clades. While targeted sequencing provides a massive quantity of data that improves phylogenetic resolution in , uncertainty still remains in sect. . The general pattern of higher site coefficient factor (CF) than gene CF in the backbone of suggests stochastic error from limited signal. Tree landscapes in combination with concordance factor scores, as implemented here, provide a comprehensive approach that incorporates several phylogenetic hypotheses. We believe the protocols employed here will be of use for future investigations on the evolutionary history of Myrtaceae.
Funder
| Funder name | Awards |
Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brazil | 155480/2018-9 - 302309/2018-7 |
American Society of Plant Taxonomists | Graduate Student Research Grant |
Royal Botanical Gardens, Kew, United Kingdom | Bentham-Moxon Trust |
Journal title
Frontiers in Plant Science
Article number
759460
Publisher
Frontiers Media SA
Place of publication
Lausanne, Switzerland
eISSN
1664-462X
Date accepted
November 29, 2021
Official URL
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In Copyright