Current status of the cryopreservation of embryogenic material of woody species.
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fpls-14-1337152.pdf
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6.37 MB
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Resource type
Journal article
Date published
January 17, 2024
Abstract
Cryopreservation, or the storage at liquid nitrogen temperatures (-196°C), of embryogenic cells or somatic embryos allows their long-term conservation without loss of their embryogenic capacity. During the last decade, protocols for cryopreservation of embryogenic material of woody species have been increasing in number and importance. However, despite the large experimental evidence proved in thousands of embryogenic lines, the application for the large-scale conservation of embryogenic material in cryobanks is still limited. Cryopreservation facilitates the management of embryogenic lines, reducing costs and time spent on their maintenance, thus limiting the risk of the appearance of somaclonal variation or contamination. Somatic embryogenesis in combination with cryopreservation is especially useful to preserve the juvenility of lines while the corresponding clones are being field-tested. Hence, when tree performance has been evaluated, selected varieties can be propagated from the cryostock. The traditional method of slow cooling or techniques based on vitrification are mostly applied procedures. For example, slow cooling methods are widely applied to conserve embryogenic lines of conifers. Desiccation based procedures, although simpler, have been applied in a smaller number of species. Genetic stability of the cryopreserved material is supported by multiloci PCR-derived markers in most of the assayed species, whereas DNA methylation status assays showed that cryopreservation might induce some changes that were also observed after prolonged subculture of the embryogenic lines. This article reviews the cryopreservation of embryogenic cultures in conifers, fruit species, deciduous forest species and palms, including a description of the different cryopreservation procedures and the analysis of their genetic stability after storage in liquid nitrogen.
Funder
| Funder name | Awards |
Ministerio de Ciencia e Innovación, Spain | PID2020-112627RB-C31 - PID2020-112627RB-C32 - PID2020-112627RB-C33 |
European Cooperation in Science and Technology | COST Action CA21157 “European Network for Innovative Woody Plant Cloning” |
Journal title
Frontiers in Plant Science
Volume
14
Article number
1337152
Publisher
Frontiers Media SA
Place of publication
Lausanne, Switzerland
eISSN
1664-462X
Date accepted
December 28, 2023
Official URL
Rights statement
In Copyright
Additional information
IF = 6.627 (2022-2023)