Morphoanatomical and phylogenetic characterization of the ectomycorrhiza between Laccaria squarrosa with Pinus pseudostrobus and its relevance for reforestation programs.
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9.94 MB
Format
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Resource type
Journal article
Date published
April 15, 2021
Abstract
Background: Pinus (Coniferophyta) and Laccaria (Basidiomycota) establish ectomycorrhizal symbioses in natural forests. However, their detailed morphoanatomical and phylogenetic characterization have received little attention. Accurate identification of native host symbionts is of paramount relevance to the production of mycorrhized seedlings for successful reforestation programs. Questions/Objective: We aimed to determine if L. squarrosa is able to establish ectomycorrhizal symbiosis with gymnosperms, thereby widening its host range and highlighting its relevance as a potential inoculant for pine seedlings. Currently, L. squarrosa is only known from its type collection associated with the angiosperm Fagus grandifolia var. mexicana. Studied species: The fungus L. squarrosa and Pinus pseudostrobus, a tree endemic to Mexico. Study site and dates: A Pinus-Quercus forest in Piedra Canteada, Nanacamilpa, Tlaxcala; 2018-2020. Methods: L. squarrosa basidiomata were identified and ectomycorrhizal roots were collected and morphoanatomically characterized. For molecular identification, DNA was extracted, PCR was performed targeting the nuclear ribosomal internal transcribed spacer region (nucrDNA ITS) for the mycobiont identification and the chloroplastic single-locus trnL region for the phytobiont. Results: In the phylogenetic analyses, our sequences from basidiomata and ectomycorrhizae clustered together with L.squarrosa with high values of supporting identity. Meanwhile, P. pseudostrobus was molecularly identified as the phytobiont. Conclusions: This is one of the few worldwide characterizations of Laccaria ectomycorrhiza under field conditions and contributes to the understanding of the ecology, distribution, and economic relevance of the symbiotic association. Our data suggest that L. squarrosa has potential for use as a native inoculant for P. pseudostrobus tree production. Translate stop Translate stop
Funder
| Funder name | Awards |
Ministry of Science and Technology of the People's Republic of China, China | China-Latin America Young Scientists Exchange Program (2017) |
Consejo Nacional de Ciencia y Tecnología, Mexico | (CONACyT)-PRONACES Food Sovereignty FOP07-2021-03 Project 316198 |
Journal title
Botanical Sciences
Publisher
Botanical Sciences, Sociedad Botanica de Mexico, AC
Place of publication
Mexico
ISSN
2007-4298
eISSN
2007-4476
Date accepted
July 9, 2021
Official URL
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In Copyright