Cryopreservation of Seeds and Seed Embryos in Orthodox-, Intermediate-, and Recalcitrant-Seeded SpeciesSeeds are one of the preferable and most used sources of germplasm for the ex situ preservation of plant genetic resources. They are generally stored dry at -20 °C in seed banks following international standards. However, some seeds do not tolerate drying and/or storage at -20 °C, or present short...
Ballesteros, Daniel ; Fanega-Sleziak, Natalia ; Davies, Rachael M.
Journal articleFern spores and pollen are haploid plant germplasm of microscopic nature that can be used to regenerate full plants through germination (fern spores) or to fertilize seed-bearing plants through breeding programs (pollen). Due to their short life span in conventional storage (i.e., dry at -20 °C), the use of cryopreservation...