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Journal article
Advances in genotyping microsatellite markers through sequencing and consequences of scoring methods for Ceratonia siliqua (Leguminosae)..
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Journal article
Phylogenetics, classification and typification of extant horsetails (Equisetum, Equisetaceae)
Horsetails (Equisetum, Equisetaceae) are one of the oldest groups of vascular plants with a nearly continuous fossil record dating back to the Carboniferous. Only a few studies have addressed relationships among and within the extant members of this fern genus. We here present the first complete phylogenetic analysis of one...Christenhusz, Maarten ; Bangiolo, Lois ; Chase, Mark ; Fay, Michael ; Husby, Chad …
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Journal article
A strong east–west Mediterranean divergence supports a new phylogeographic history of the carob tree (Ceratonia siliqua, Leguminosae) and multiple domestications from native populations
Aim Phylogeography of fruit trees is challenging due to recurrent exchanges between domesticated and wild populations. Here we tested the eastern refugium hypothesis (ERH) for the carob tree, Ceratonia siliqua, which supports its natural and domestication origins in the eastern Mediterranean and a feral origin in the west. Location... -
Journal article
A Target Capture-Based Method to Estimate Ploidy From Herbarium Specimens
Whole genome duplication (WGD) events are common in many plant lineages, but the ploidy status and possible occurrence of intraspecific ploidy variation are unknown for most species. Standard methods for ploidy determination are chromosome counting and flow cytometry approaches. While flow cytometry approaches typically use fresh tissue, an increasing number...Viruel, Juan ; Conejero, María ; Hidalgo, Oriane ; Pokorny, Lisa ; Powell, Robyn …
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Journal article
A customized nuclear target enrichment approach for developing a phylogenomic baseline for Dioscorea yams (Dioscoreaceae)
Premise We developed a target enrichment panel for phylogenomic studies of Dioscorea, an economically important genus with incompletely resolved relationships. Methods Our bait panel comprises 260 low- to single-copy nuclear genes targeted to work in Dioscorea, assessed here using a preliminary taxon sampling that includes both distantly and closely related...Soto Gomez, Marybel ; Pokorny, Lisa ; Kantar, Michael ; Forest, Félix ; Leitch, Ilia …
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Journal article
Phenolic Compounds Content and Genetic Diversity at Population Level across the Natural Distribution Range of Bearberry (Arctostaphylos uva-ursi, Ericaceae) in the Iberian Peninsula
Bearberry (Arctostaphylos uva-ursi) is a medicinal plant traditionally employed for the treatment of urinary tract infections due to high contents of arbutin (hydroquinone β-D-glucoside), which is now mainly used as a natural skin-whitening agent in cosmetics. Bearberry has also been proposed as a natural antioxidant additive due to the high...Asensio, Esther ; Vitales, Daniel ; Pérez, Iván ; Peralba, Laia ; Viruel, Juan …
haplotypes, arbutin, natural antioxidants, genome size, and genetic and phytochemical variability
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Journal article
Microsatellite marker development in the crop wild relative Linum bienne using genome skimming
Premise: Nuclear microsatellite markers were developed for Linum bienne, the sister species of the crop L. usitatissimum, to provide molecular genetic tools for the investigation of L. bienne genetic diversity and structure. Methods and Results: Fifty microsatellite loci were identified in L. bienne by means of genome skimming, and 44...Landoni, Beatrice ; Viruel, Juan ; Gómez, Rocio ; Allaby, Robin G. ; Brennan, Adrian C. …
Linum bienne, pale flax, population genetics, crop wild relative, Linaceae, and simple sequence repeat (SSR)
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Journal article
Strategies for reducing per‐sample costs in target capture sequencing for phylogenomics and population genomics in plants
The reduced cost of high‐throughput sequencing and the development of gene sets with wide phylogenetic applicability has led to the rise of sequence capture methods as a plausible platform for both phylogenomics and population genomics in plants. An important consideration in large targeted sequencing projects is the per‐sample cost, which...
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