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Journal article
Storage of orchid pollinia with varying lipid thermal fingerprints
Orchid pollinia have the potential to make a valuable contribution to current techniques of germplasm storage and assisted reproduction, yet information regarding their preservation and their ability to remain viable over time is currently limited. Dactylorhiza fuchsii and Disa uniflora were used as models for investigating potential techniques for storing...Custodio, Ceci Castilho ; Machado-Neto, Nelson B. ; Singer, Rodrigo B. ; Pritchard, Hugh W. ; Seaton, Philip T. …
Orchidaceae, Conservation , Germplasm, and Pollen germination
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Journal article
Comparative Seed Morphology of Tropical and Temperate Orchid Species with Different Growth Habits
Seed morphology underpins many critical biological and ecological processes, such as seed dormancy and germination, dispersal, and persistence. It is also a valuable taxonomic trait that can provide information about plant evolution and adaptations to different ecological niches. This study characterised and compared various seed morphological traits, i.e., seed and...Diantina, Surya ; McGill, Craig ; Millner, James ; Nadarajan, Jayanthi ; Pritchard, Hugh W. …
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Journal article
Lack of adequate seed supply is a major bottleneck for effective ecosystem restoration in Chile: friendly amendment to Bannister et al. (2018)
We argue that the need for a quality seed supply chain is a major bottleneck for the restoration of Chile's native ecosystems, thus supplementing the list of bottlenecks proposed by Bannister et al. in 2018. Specifically, there is a need for defining seed transfer zones, developing standards and capacities for...León‐Lobos, Pedro ; Bustamante‐Sánchez, Marcela A. ; Nelson, Cara R. ; Alarcón, Diego ; Hasbún, Rodrigo …
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Journal article
Dry architecture: towards the understanding of the variation of longevity in desiccation-tolerant germplasm
Desiccation-tolerant (DT) plant germplasm (i.e. seeds, pollen and spores) survive drying to low moisture contents, when cytoplasm solidifies, forming a glass, and chemical reactions are slowed. DT germplasm may survive for long periods in this state, though inter-specific and intra-specific variation occurs and is not currently explained. Such variability has...Ballesteros, Daniel ; Pritchard, Hugh W. ; Walters, Christina
solidified cytoplasm, seeds, oxidative stress, photosynthetic stress, spores, and lipid crystallization
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Journal article
Seed Survival at Low Temperatures: A Potential Selecting Factor Influencing Community Level Changes in High Altitudes under Climate Change
In alpine ecosystems, imbibed seeds are often exposed to temperatures as low as −35 °C, challenging their survival in the soil. Here, we show that seeds have mechanisms to survive cold climate prevalent in alpine ecosystems and have identified three such mechanisms from existing literature, including two forms of freezing avoidance...Jaganathan, Ganesh K. ; Dalrymple, Sarah E. ; Pritchard, Hugh W.
Boreal, supercooling, freezing tolerance, freezing avoidance, and species migration
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Journal article
Comparative in vitro seed germination and seedling development in tropical and temperate epiphytic and temperate terrestrial orchids
Ex situ conservation of orchid seeds requires the application of modern biotechnology to determine seed quality and to optimise in vitro regeneration. The nutrient requirements for germination and seedling growth in vitro can be species-specific and influenced by life history traits and habitats. Therefore, this study aimed to explore in... -
Journal article
The negative effect of a vertically-transmitted fungal endophyte on seed longevity is stronger than that of ozone transgenerational effect
Seed morphology underpins many critical biological and ecological processes, such as seed dormancy and germination, dispersal, and persistence. It is also a valuable taxonomic trait that can provide information about plant evolution and adaptations to different ecological niches. This study characterised and compared various seed morphological traits, i.e., seed and...Dantas, Barbara F. ; Moura, Magna S. B. ; Gundel, Pedro E. ; McGill, Craig ; Seal, Charlotte E. …
Maternal effect, Lolium multiflorum, Italian ryegrass, and Epichloë occultans