Search Constraints
Search Results
-
2019
Journal article
Polyploidy in the Conifer Genus Juniperus: An Unexpectedly High Rate
Recent research suggests that the frequency of polyploidy may have been underestimated in gymnosperms. While there are few reports of polyploids in the genus Juniperus, data are still missing for most species. In this study, we evaluated the extent of polyploidy in Juniperus by conducting the first comprehensive screen across...Farhat, Perla ; Hidalgo, Oriane ; Robert, Thierry ; Siljak-Yakovlev, Sonja ; Leitch, Ilia …
-
2019
Journal article
A Target Capture-Based Method to Estimate Ploidy From Herbarium Specimens
Whole genome duplication (WGD) events are common in many plant lineages, but the ploidy status and possible occurrence of intraspecific ploidy variation are unknown for most species. Standard methods for ploidy determination are chromosome counting and flow cytometry approaches. While flow cytometry approaches typically use fresh tissue, an increasing number...Viruel, Juan ; Conejero, María ; Hidalgo, Oriane ; Pokorny, Lisa ; Powell, Robyn …
-
2019
Journal article
Factors Affecting Targeted Sequencing of 353 Nuclear Genes From Herbarium Specimens Spanning the Diversity of Angiosperms
The world’s herbaria collectively house millions of diverse plant specimens, including endangered or extinct species and type specimens. Unlocking genetic data from the typically highly degraded DNA obtained from herbarium specimens was difficult until the arrival of high-throughput sequencing approaches, which can be applied to low quantities of severely fragmented...