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Journal article
Solid-State Biology and Seed Longevity: A Mechanical Analysis of Glasses in Pea and Soybean Embryonic Axes
The cytoplasm of anhydrobiotes (organisms that persist in the absence of water) solidifies during drying. Despite this stabilization, anhydrobiotes vary in how long they persist while dry. In this paper, we call upon concepts currently used to explain stability of amorphous solids (also known as glasses) in synthetic polymers, foods,...Ballesteros, Daniel ; Walters, Christina
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Journal article
Survival and growth of embryo axes of temperate trees after two decades of cryo-storage
Liquid nitrogen (LN) storage is recommended for conserving plants with seeds for which standard seed banking is not feasible, but there are few empirical reports confirming its long-term effectiveness. In this study we evaluated in vitro viability and ex vitro growth of embryo axes of a species that is short-lived...Ballesteros, Daniel ; Pence, Valerie
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Journal article
Desiccation Tolerance in Chlorophyllous Fern Spores: Are Ecophysiological Features Related to Environmental Conditions?
Fern spores of most species are desiccation tolerant (DT) and, in some cases, are photosynthetic at maturation, the so-called chlorophyllous spores (CS). The lifespan of CS in the dry state is very variable among species. The physiological, biochemical, and biophysical mechanisms underpinning this variability remain understudied and their interpretation from... -
Journal article
Dry architecture: towards the understanding of the variation of longevity in desiccation-tolerant germplasm
Desiccation-tolerant (DT) plant germplasm (i.e. seeds, pollen and spores) survive drying to low moisture contents, when cytoplasm solidifies, forming a glass, and chemical reactions are slowed. DT germplasm may survive for long periods in this state, though inter-specific and intra-specific variation occurs and is not currently explained. Such variability has...Ballesteros, Daniel ; Pritchard, Hugh W. ; Walters, Christina
solidified cytoplasm, seeds, oxidative stress, photosynthetic stress, spores, and lipid crystallization
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Journal article
Cryopreservation of Fern Spores and Pollen
Fern spores and pollen are haploid plant germplasm of microscopic nature that can be used to regenerate full plants through germination (fern spores) or to fertilize seed-bearing plants through breeding programs (pollen). Due to their short life span in conventional storage (i.e., dry at -20 °C), the use of cryopreservation... -
Journal article
Cryopreservation of Seeds and Seed Embryos in Orthodox-, Intermediate-, and Recalcitrant-Seeded Species
Seeds are one of the preferable and most used sources of germplasm for the ex situ preservation of plant genetic resources. They are generally stored dry at -20 °C in seed banks following international standards. However, some seeds do not tolerate drying and/or storage at -20 °C, or present short... -
Journal article
The Cryobiotechnology of Oaks: An Integration of Approaches for the Long-Term Ex Situ Conservation of Quercus Species
Conventional dry seed storage is unlikely for about one third of all tree species (and nearly half of evergreen rain forest trees) as they probably produce desiccation sensitive (recalcitrant) seeds. Consequently, international ex situ conservation targets for threatened trees will be difficult to achieve without innovation, especially in cryobiotechnology. We...Ballesteros, Daniel ; Pritchard, Hugh W.
recalcitrant seeds, cryopreservation, pollen, embryonic axes, DOE, tree genetic resources, shoot tips, in vitro culture, and dormant buds
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Journal article
Survival and genetic stability of shoot tips of Hedeoma todsenii after long-term cryostorage.
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