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2022
Journal article
Bryophyte Spores Tolerate High Desiccation Levels and Exposure to Cryogenic Temperatures but Contain Storage Lipids and Chlorophyll: Understanding the Essential Traits Needed for the Creation of Bryophyte Spore Banks.
Understanding the desiccation and freezing tolerance of bryophyte spores is vital to explain how plants conquered land and current species distribution patterns and help to develop efficient ex situ conservation methods. However, knowledge of these traits is scarce. We investigated tolerance to drying (at 15% relative humidity [RH] for two... -
2022
Journal article
Does oxygen affect ageing mechanisms of Pinus densiflora seeds? A matter of cytoplasmic physical state.
During desiccation, the cytoplasm of orthodox seeds solidifies into an intracellular glass with highly restricted diffusion and molecular mobility. Temperature and water content govern seed ageing rates, while oxygen (O2) can promote deteriorative reactions. However, whether the cytoplasmic physical state affects involvement of O2 in seed ageing remains unresolved. We... -
2021
Journal article
Plant Diversity Conservation Challenges and Prospects—The Perspective of Botanic Gardens and the Millennium Seed Bank.
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2017
Journal article
Survival and genetic stability of shoot tips of Hedeoma todsenii after long-term cryostorage.
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2020
Journal article
The Cryobiotechnology of Oaks: An Integration of Approaches for the Long-Term Ex Situ Conservation of Quercus Species
Conventional dry seed storage is unlikely for about one third of all tree species (and nearly half of evergreen rain forest trees) as they probably produce desiccation sensitive (recalcitrant) seeds. Consequently, international ex situ conservation targets for threatened trees will be difficult to achieve without innovation, especially in cryobiotechnology. We...Ballesteros, Daniel ; Pritchard, Hugh W.
recalcitrant seeds, cryopreservation, pollen, embryonic axes, DOE, tree genetic resources, shoot tips, in vitro culture, and dormant buds
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2020
Journal article
Cryopreservation of Seeds and Seed Embryos in Orthodox-, Intermediate-, and Recalcitrant-Seeded Species
Seeds are one of the preferable and most used sources of germplasm for the ex situ preservation of plant genetic resources. They are generally stored dry at -20 °C in seed banks following international standards. However, some seeds do not tolerate drying and/or storage at -20 °C, or present short...