Search Constraints
Search Results
-
2024
Journal article
Current status of the cryopreservation of embryogenic material of woody species.
Cryopreservation, or the storage at liquid nitrogen temperatures (-196°C), of embryogenic cells or somatic embryos allows their long-term conservation without loss of their embryogenic capacity. During the last decade, protocols for cryopreservation of embryogenic material of woody species have been increasing in number and importance. However, despite the large experimental... -
2024
Journal article
Plant Cryopreservation: Principles, Applications, and Challenges of Banking Plant Diversity At Ultralow Temperatures..
-
2023
Journal article
Seed Longevity—The Evolution of Knowledge and a Conceptual Framework.
The lifespan or longevity of a seed is the time period over which it can remain viable. Seed longevity is a complex trait and varies greatly between species and even seed lots of the same species. Our scientific understanding of seed longevity has advanced from anecdotal ‘Thumb Rules,’ to empirically...Nadarajan, Jayanthi ; Walters, Christina ; Pritchard, Hugh W. ; Ballesteros, Daniel ; Colville, Louise
Cryopreservation, Seed longevity, Glassy state, Cryobiotechnology, and Seed ageing
-
2022
Journal article
Humidity and Light Modulate Oxygen-Induced Viability Loss in Dehydrated Haematococcus lacustris Cells.
Haematoccocus lacustris (previously H. pluvialis) is a desiccation-tolerant unicellular freshwater green alga. During acclimation to desiccation, astaxanthin-rich lipid bodies and low-molecular-weight antioxidants (α-tocopherol, glutathione) accumulate, while the chloroplast area and chlorophyll contents decrease, which may facilitate desiccation tolerance by preventing damage mediated by reactive oxygen species (ROS). Here, we investigated... -
2022
Journal article
Bryophyte Spores Tolerate High Desiccation Levels and Exposure to Cryogenic Temperatures but Contain Storage Lipids and Chlorophyll: Understanding the Essential Traits Needed for the Creation of Bryophyte Spore Banks.
Understanding the desiccation and freezing tolerance of bryophyte spores is vital to explain how plants conquered land and current species distribution patterns and help to develop efficient ex situ conservation methods. However, knowledge of these traits is scarce. We investigated tolerance to drying (at 15% relative humidity [RH] for two... -
2022
Journal article
Does oxygen affect ageing mechanisms of Pinus densiflora seeds? A matter of cytoplasmic physical state.
During desiccation, the cytoplasm of orthodox seeds solidifies into an intracellular glass with highly restricted diffusion and molecular mobility. Temperature and water content govern seed ageing rates, while oxygen (O2) can promote deteriorative reactions. However, whether the cytoplasmic physical state affects involvement of O2 in seed ageing remains unresolved. We...