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Filtering by: Institution Royal Botanic Gardens, Kew Research Repository Creator Dodsworth, Steven Creator Pokorny, Lisa Keyword Hyb-Seq

2018

Journal article

A Universal Probe Set for Targeted Sequencing of 353 Nuclear Genes from Any Flowering Plant Designed Using k-Medoids Clustering

Sequencing of target-enriched libraries is an efficient and cost-effective method for obtaining DNA sequence data from hundreds of nuclear loci for phylogeny reconstruction. Much of the cost of developing targeted sequencing approaches is associated with the generation of preliminary data needed for the identification of orthologous loci for probe design....

Johnson, Matthew G. ; Pokorny, Lisa ; Dodsworth, Steven ; Botigué, Laura R. ; Cowan, Robyn S. …
sequence capture, nuclear genes, phylogenomics, angiosperms, machine learning, k-means clustering, target enrichment, Hyb-Seq, and k-medoids clustering
2019

Journal article

Hyb-Seq for Flowering Plant Systematics

High-throughput DNA sequencing (HTS) presents great opportunities for plant systematics, yet genomic complexity needs to be reduced for HTS to be effectively applied. We highlight Hyb-Seq as a promising approach, especially in light of the recent development of probes enriching 353 low-copy nuclear genes from any flowering plant taxon.

Dodsworth, Steven ; Pokorny, Lisa ; Johnson, Matthew ; Kim, Jan ; Maurin, Olivier …
phylogenetics, molecular systematics, sequence capture, angiosperms, genomics, tree of life, high-throughput sequencing, and Hyb-Seq