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Search Results
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2018
Journal article
A Universal Probe Set for Targeted Sequencing of 353 Nuclear Genes from Any Flowering Plant Designed Using k-Medoids Clustering
Sequencing of target-enriched libraries is an efficient and cost-effective method for obtaining DNA sequence data from hundreds of nuclear loci for phylogeny reconstruction. Much of the cost of developing targeted sequencing approaches is associated with the generation of preliminary data needed for the identification of orthologous loci for probe design.... -
2018
Journal article
Earth BioGenome Project: Sequencing life for the future of life
Increasing our understanding of Earth’s biodiversity and responsibly stewarding its resources are among the most crucial scientific and social challenges of the new millennium. These challenges require fundamental new knowledge of the organization, evolution, functions, and interactions among millions of the planet’s organisms. Herein, we present a perspective on the...Lewin, Harris A. ; Robinson, Gene E. ; Kress, W. John ; Baker, William J. ; Coddington, Jonathan …
genome sequencing, data science, biodiversity, genomics, and access and benefit sharing
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2018
Journal article
A roadmap for global synthesis of the plant tree of life
Providing science and society with an integrated, up‐to‐date, high quality, open, reproducible and sustainable plant tree of life would be a huge service that is now coming within reach. However, synthesizing the growing body of DNA sequence data in the public domain and disseminating the trees to a diverse audience... -
2019
Journal article
A phylogenomic analysis of Nepenthes (Nepenthaceae)
Nepenthaceae is one of the largest carnivorous plant families and features ecological and morphological adaptations indicating an impressive adaptive radiation. However, investigation of evolutionary and taxonomic questions is hindered by poor phylogenetic understanding, with previous molecular studies based on limited loci and taxa. We use high-throughput sequencing with a target-capture...Murphy, Bruce ; Forest, Félix ; Barraclough, Timothy ; Rosindell, James ; Bellot, Sidonie …
Genetics, Molecular Biology, and Ecology, Evolution, Behavior and Systematics
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2019
Journal article
Hyb-Seq for Flowering Plant Systematics
High-throughput DNA sequencing (HTS) presents great opportunities for plant systematics, yet genomic complexity needs to be reduced for HTS to be effectively applied. We highlight Hyb-Seq as a promising approach, especially in light of the recent development of probes enriching 353 low-copy nuclear genes from any flowering plant taxon. -
2020
Journal article
Reconstructing the Complex Evolutionary History of the Papuasian Schefflera Radiation Through Herbariomics
Shee, Zhi Qiang ; Frodin, David G. ; Cámara-Leret, Rodrigo ; Pokorny, Lisa
Schefflera, Sequence capture, Historical biogeography, Araliaceae, Target enrichment, Papuasia, New Guinea, and Herbariomics
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2021
Journal article
Combination of Sanger and target-enrichment markers supports revised generic delimitation in the problematic ‘Urera clade’ of the nettle family (Urticaceae)
Urera Gaudich, s.l. is a pantropical genus comprising c. 35 species of trees, shrubs, and vines. It has a long history of taxonomic uncertainty, and is repeatedly recovered as polyphyletic within a poorly resolved complex of genera in the Urticeae tribe of the nettle family (Urticaceae). To provide generic delimitations...Wells, Tom ; Maurin, Olivier ; Dodsworth, Steven ; Friis, Ib ; Cowan, Robyn …
Lianas, Secondary woodiness, Generic delimitation, and Angiosperms353
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2021
Journal article
Phylogenomics and biogeography of Cunoniaceae (Oxalidales) with complete generic sampling and taxonomic realignments.
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2021
Journal article
Resolving generic limits in Cyperaceae tribe Abildgaardieae using targeted sequencing
Morphological characterizations of genera in Cyperaceae tribe Abildgaardieae have been highly problematic and the subject of much debate. Earlier molecular phylogenetic studies based on Sanger sequencing and a limited sampling have indicated that several generic circumscriptions are not monophyletic. Here, we provide the first phylogenetic hypothesis for Abildgaardieae using targeted...Larridon, Isabel ; Zuntini, Alexandre R. ; Barrett, Russell L. ; Wilson, Karen L. ; Bruhl, Jeremy J. …
Fimbristylis, C4 photosynthesis, generic circumscription, Bulbostylis, and Angiosperms353
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2021