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Journal article
A Target Capture-Based Method to Estimate Ploidy From Herbarium Specimens
Whole genome duplication (WGD) events are common in many plant lineages, but the ploidy status and possible occurrence of intraspecific ploidy variation are unknown for most species. Standard methods for ploidy determination are chromosome counting and flow cytometry approaches. While flow cytometry approaches typically use fresh tissue, an increasing number...Viruel, Juan ; Conejero, María ; Hidalgo, Oriane ; Pokorny, Lisa ; Powell, Robyn …
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Journal article
Polyploidy does not control all: Lineage‐specific average chromosome length constrains genome size evolution in ferns
Recent studies investigating the evolution of genome size diversity in ferns have shown that they have a distinctive genome profile compared with other land plants. Ferns are typically characterized by possessing medium‐sized genomes, although a few lineages have evolved very large genomes. Ferns are different from other vascular plant lineages...Liu, Hong‐Mei ; Ekrt, Libor ; Koutecky, Petr ; Pellicer, Jaume ; Hidalgo, Oriane …
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Journal article
A customized nuclear target enrichment approach for developing a phylogenomic baseline for Dioscorea yams (Dioscoreaceae)
Premise We developed a target enrichment panel for phylogenomic studies of Dioscorea, an economically important genus with incompletely resolved relationships. Methods Our bait panel comprises 260 low- to single-copy nuclear genes targeted to work in Dioscorea, assessed here using a preliminary taxon sampling that includes both distantly and closely related...Soto Gomez, Marybel ; Pokorny, Lisa ; Kantar, Michael ; Forest, Félix ; Leitch, Ilia …
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Journal article
Do tropical plants have smaller genomes? Correlation between genome size and climatic variables in the Caesalpinia Group (Caesalpinioideae, Leguminosae)
While a number of studies have suggested that temperate plants typically have larger genome sizes (GS) than tropical ones, recent analyses have not convincingly confirmed this. We have selected the widely distributed Caesalpinia Group (Leguminosae) to test this hypothesis. We used flow cytometry to estimate the amount of DNA in...Souza, Gustavo ; Costa, Lucas ; Guignard, Maïté ; Van-Lume, Brena ; Pellicer, Jaume …
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Journal article
Early consequences of allopolyploidy alter floral evolution in Nicotiana (Solanaceae)
Background: Polyploidy has played a major role in angiosperm evolution. Previous studies have examined polyploid phenotypes in comparison to their extant progenitors, but not in context of predicted progenitor phenotypes at allopolyploid origin. In addition, differences in the trends of polyploid versus diploid evolution have not been investigated. We use...McCarthy, E ; Landis, J ; Kurti, A ; Lawhorn, A ; Chase, M …
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Journal article
Interactions between plant genome size, nutrients and herbivory by rabbits, molluscs and insects on a temperate grassland
Angiosperm genome sizes (GS) vary ca 2400-fold. Recent research has shown that GS influences plant abundance, and plant competition. There are also tantalizing reports that herbivores may select plants as food dependent on their GS. To test the hypothesis that GS plays a role in shaping plant communities under herbivore...Guignard, Maïté ; Crawley, Michael ; Kovalenko, Dasha ; Nichols, Richard ; Trimmer, Mark …
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Journal article
Polyploidy in the Conifer Genus Juniperus: An Unexpectedly High Rate
Recent research suggests that the frequency of polyploidy may have been underestimated in gymnosperms. While there are few reports of polyploids in the genus Juniperus, data are still missing for most species. In this study, we evaluated the extent of polyploidy in Juniperus by conducting the first comprehensive screen across...Farhat, Perla ; Hidalgo, Oriane ; Robert, Thierry ; Siljak-Yakovlev, Sonja ; Leitch, Ilia …
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Journal article
Remarkable variation of ribosomal DNA organization and copy number in gnetophytes, a distinct lineage of gymnosperms
INTRODUCTION: Gnetophytes, comprising the genera Ephedra, Gnetum and Welwitschia, are an understudied, enigmatic lineage of gymnosperms with a controversial phylogenetic relationship to other seed plants. Here we examined the organization of ribosomal DNA (rDNA) across representative species. METHODS: We applied high-throughput sequencing approaches to isolate and reconstruct rDNA units and...Wang, W ; Wan, T ; Becher, H ; Kuderova, A ; Leitch, Ilia …